Novel in situ seeding immunodetection assay uncovers neuronal-driven alpha-synuclein seeding in Parkinson's disease.

ABSTRACT: Aggregates of alpha-synuclein (α-syn) propagate through template-induced misfolding in people with Parkinson's disease (PD) and Multiple System Atrophy (MSA). Prion-like seeding is crucial in disease initiation and progression, representing a major target for disease-modifying therapies. The detection of α-syn seeding with seeding amplification assays (SAAs) has remarkable diagnostic and research potential. However, current SAAs rely on bulk tissue homogenates or fluids, losing critical spatial and cellular resolution. Here, we report our novel in situ seeding immunodetection (isSID) assay that enables the visualization of seeding with unprecedented morphological detail in intact biological tissue. Using the isSID assay, we confirm seeding activity in α-syn aggregates in PD, MSA, and other

SUBMITTER: Otero-Jimenez M 

PROVIDER: S-EPMC12379234 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature