Investigating the role of molecular coating in human corneal endothelial cell primary culture using artificial intelligence-driven image analysis.

ABSTRACT: The monolayer of approximately 300,000 human corneal endothelial cells (hCECs) on the posterior surface of the cornea is essential to maintain transparency but is non-self-regenerative. Corneal blindness can currently only be treated by corneal transplantation, hindered by a global donor shortage, highlighting the need for developing tissue and/or cell therapy. The mass production of these advanced therapy medicinal products requires obtaining high-yield, high-quality endothelial cell cultures characterized by hexagonal shape, low size variability, and high endothelial cell density (ECD). Among the usual critical quality attributes which combine the expression of differentiation markers, ECD and cell morphological parameters, the latter are not optimally measured in vitro by conventional i

SUBMITTER: Travers G 

PROVIDER: S-EPMC12379294 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature