Construction of a portable multiplex detection system for four bee viral paralysis diseases based on RT-PCR-microfluidic chip integrated technology.

ABSTRACT:

Background

As crucial pollinators sustaining agricultural ecosystem services and biodiversity, bees mediate pollination for approximately 35% of global insect-pollinated crops and generate multidimensional ecological value through apicultural products in the pharmaceutical and food industries. However, emerging viral pathogens pose escalating threats to bee health.

Results

To address the technical bottlenecks in pathogen detection for viral paralysis disease in bees, this study innovatively integrated multiplex RT-PCR amplification, lateral flow dipstick (LFD), and centrifugal microfluidic chip technology (MFCT) to develop an on-site quadruple detection platform capable of simultaneously identifying four viruses: Chronic Bee Paralysis Virus (CBPV), Black Queen Cell Virus (BQ

SUBMITTER: Wang Z 

PROVIDER: S-EPMC12379352 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature