Molecular analysis of androgen receptor splice variant AR-V3 reveals eminent ambiguity regarding activity and clinical utility.

ABSTRACT: Androgen receptor (AR) splice variants (AR-Vs) have emerged as potential resistance mechanisms to AR-targeted agents (ARTAs) in prostate cancer (PC), particularly in the context of castration-resistant disease. Among them, AR-V3 has been frequently detected, yet its biological function remains unclear due to conflicting results from initial studies. This study aimed to comprehensively investigate the molecular structure, activity, and clinical relevance of AR-V3. We constructed plasmids encoding two AR-V3 isoforms-one newly identified isoform matching the human reference genome (AR-V3ref) and the other based on the 22Rv1 cell line sequence (AR-V322Rv1)-and transfected them into AR-negative PC-3 cells alone or co-expressed with AR full-length (AR-FL). Localization, transcriptional activity

SUBMITTER: Wustmann N 

PROVIDER: S-EPMC12379386 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature