Establishment of a CRISPR/Cas9 gene editing system based on growth points transformation method in Fraxinus mandshurica.

ABSTRACT: The lack of an effective gene editing technology system for Fraxinus mandshurica makes it challenging to improve its traits through genetic engineering methods. In this study, an effective CRISPR/Cas9 gene editing system targeting plant growth points was established through the optimization of Agrobacterium tumefaciens concentration and infection duration. Furthermore, a tissue culture system for clustered buds was developed by supplementing the media with hormones at different concentrations. FmbHLH1-edited chimeric plants were successfully generated using the developed CRISPR/Cas9 gene editing system. Homozygous plants were induced and screened using the developed clustered bud system. Among 100 randomly transformed growing points, 18% of the induced clustered buds were gene-edited, whic

SUBMITTER: Li L 

PROVIDER: S-EPMC12379479 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature