TDO-mediated tissue-specific regulation of kynurenine pathway in the brain and gill of Litopenaeus vannamei.

ABSTRACT: The kynurenine pathway (KP), central to tryptophan metabolism, regulates neuroimmune interactions in vertebrates through the dual actions of indoleamine 2,3-dioxygenase (IDO) and tryptophan 2,3-dioxygenase (TDO). However, crustaceans lack IDO and rely solely on TDO, yet the tissue-specific roles of TDO in this lineage remain unknown. Using Litopenaeus vannamei, a key aquaculture species, we investigated how TDO-mediated KP adapts to functional constraints in neural and respiratory tissues. Genomic analyses confirmed the absence of IDO in crustaceans, while LvTDO retained catalytic domains homologous to vertebrates but acquired lineage-specific structural features. Tissue-specific profiling revealed dominant LvTDO expression in brain and gill. RNAi knockdown triggered distinct responses: th

SUBMITTER: Tan G 

PROVIDER: S-EPMC12379511 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature