Monitoring SARS-CoV-2 variants in wastewater during periods of low clinical case surveillance in Ethiopia.

ABSTRACT: Wastewater-based genomic surveillance is a cost-effective approach for tracking outbreaks like severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A 2023 study in Addis Ababa, Ethiopia, revealed two infection waves in March and August, with the latter undetected by clinical surveillance. This study analyzed the viral spread and evolution in the population during periods of low reported cases. Viral concentration was performed following the Ceres Technology protocol, and RNA was extracted using the QIAamp Viral RNA Mini Kit. Quantitative PCR was performed using the TaqPath COVID-19 Kit. Samples with cycle threshold values ≤32 were used for sequencing. Library preparation and sequencing were performed using the Illumina COVIDSeq protocol, and data analysis was conducted using the F

SUBMITTER: Gebremicael G 

PROVIDER: S-EPMC12379605 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature