Development of an automated 3D high content cell screening platform for organoid phenotyping.

ABSTRACT: The use of organoid models in biomedical research has grown substantially since their inception. As they gain popularity among scientists seeking more complex and biologically relevant systems, there is a direct need to expand and clarify potential uses of such systems in diverse experimental contexts. Herein we outline a high-content screening (HCS) platform that allows researchers to screen drugs or other compounds against three-dimensional (3D) cell culture systems in a multi-well format (384-well). Furthermore, we compare the quality of robotic liquid handling with manual pipetting and characterize and contrast the phenotypic effects detected by confocal imaging and biochemical assays in response to drug treatment. We show that robotic liquid handling is more consistent and amendable t

SUBMITTER: Bozal SB 

PROVIDER: S-EPMC12380041 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature