Time-Lapse Tracking of Acanthamoeba castellanii Trophozoite Activity During Exposure to Contact Lens Disinfectants.

ABSTRACT: To compare the immediate response of Acanthamoeba castellanii trophozoites to the sudden exposure of common contact lens disinfectants by tracking movement behavior through time-lapse imaging. We cultured Acanthamoeba trophozoites and imaged them under brightfield microscopy. We introduced disinfectants at commercially relevant concentrations into the environment of the trophozoites for 30 minutes. After exposure, we neutralized the disinfectants and returned the samples to a growth medium for incubation. We converted recordings into binary image sequences and analyzed trophozoite movement and body morphology using cell-tracking software. After placing amoebae in a growth medium, we assessed recovery at 1 day and 3 days post-exposure. Exposure to 0.05% w/v povidone-iodine (PVP-I) resulted

SUBMITTER: Bahr C 

PROVIDER: S-EPMC12380112 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature