Aspergillus fumigatus viability drives allergic responses to inhaled conidia.

ABSTRACT:

Background

Aspergillus fumigatus-induced allergic airway disease has been shown to involve conidial germination in vivo, but the immunological mechanisms remain uncharacterized.

Objective

A subchronic murine exposure model was used to examine the immunological mediators that are regulated in response to either culturable or nonculturable A fumigatus conidia.

Methods

Female B6C3F1/N mice were repeatedly dosed via inhalation with 1 × 105 viable or heat-inactivated conidia (HIC), twice per week for 13 weeks (26 exposures). Control mice inhaled high-efficiency particulate arrestor-filtered air. The influence of A fumigatus conidial germination on the pulmonary immunopathological outcomes was evaluated by flow cytometry analysis of cellular infiltration in the airways, ass

SUBMITTER: Nayak AP 

PROVIDER: S-EPMC12380257 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature