Establishment of reverse transcription recombinase-aided amplification with lateral flow dipstick for the rapid visual detection of Getah virus.

ABSTRACT:

Introduction

Getah virus (GETV) is a globally spreading zoonotic mosquito-borne virus that primarily affects horses and pigs, causing significant economic losses in the livestock industry. Therefore, there is an urgent need for improved diagnostic methods to manage future outbreaks.

Methods

In this study, we developed a nucleic acid detection assay, reverse transcription recombinase-aided amplification with lateral flow dipstick (RT-RAA-LFD), for the rapid and convenient detection of GETV.

Results

The RT-RAA-LFD assay could be completed at 40°C for 15 min. Under optimal reaction conditions, the assay demonstrated excellent specificity, with no cross-reactivity observed with other clinically relevant swine pathogens. It achieved a broad detection range and a limit of d

SUBMITTER: Wei B 

PROVIDER: S-EPMC12380932 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature