Role of N6-methyladenosine methylation in transverse aortic constriction-induced cardiac fibrosis: insights from MeRIP-seq analysis.

ABSTRACT: This study aimed to investigate the role and potential mechanisms of N6-methyladenosine (m6A) methylation in a mouse model of transverse aortic constriction (TAC)-induced cardiac fibrosis using MeRIP-seq. A TAC-induced cardiac fibrosis mouse model was established, and cardiac function and structural parameters were assessed by echocardiography four weeks post-surgery. The global m6A methylation levels in myocardial tissues were evaluated using Dot blot analysis, and the expression levels of m6A-modifying enzymes (METTL3, METTL14, ALKBH5, FTO) were detected by qPCR and Western blot. Additionally, methylated RNA immunoprecipitation sequencing (MeRIP-Seq) was performed to identify differentially methylated sites, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG

SUBMITTER: Liu S 

PROVIDER: S-EPMC12380991 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature