Effect of miR-6767-5p on breast cancer cell phenotype and its regulatory mechanism.

ABSTRACT: Objective To investigate the role and mechanism of miR-6767-5p in breast cancer (BC). We explored the effects of miR-6767-5p on the proliferation, migration, and invasion of BC cells in vitro and in vivo through CCK-8, EdU, Transwell, and subcutaneous tumorigenesis experiments in nude mice and a tail vein lung metastasis model. Cysteine-rich intestinal protein 2 (CRIP2) was validated as a target gene of miR-6767-5p through dual-luciferase reporter assays, quantitative polymerase chain reaction (qPCR), and western blot (WB) analysis. WB was conducted to investigate the impact of miR-6767-5p on the NF-κB signaling pathway and its association with the epithelial‒mesenchymal transition (EMT). Coimmunoprecipitation, chromatin immunoprecipitation, qPCR and WB were used to verify the possible rel

SUBMITTER: Tan ZH 

PROVIDER: S-EPMC12381055 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature