DeFrND: detergent-free reconstitution into native nanodiscs with designer membrane scaffold peptides.

ABSTRACT: Membrane scaffold protein-based nanodiscs have facilitated unprecedented structural and biophysical analysis of membrane proteins in a near-native lipid environment. However, successful reconstitution of membrane proteins in nanodiscs requires prior solubilization and purification in detergents, which may impact their physiological structure and function. Furthermore, the detergent-mediated reconstitution of nanodiscs is unlikely to recapitulate the precise composition or asymmetry of native membranes. To circumvent this fundamental limitation of traditional nanodisc technology, we herein describe the development of membrane-solubilizing peptides to directly extract membrane proteins from native cell membranes into nanoscale discoids. By systematically protein engineering and screening, we

SUBMITTER: Ren Q 

PROVIDER: S-EPMC12381110 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature