Hypoxia suppressed the Siglec-5 signaling in TAMs via modulating the balance of SHP2/SYK activation in hepatocellular carcinoma.

ABSTRACT:

Objective

To investigate the effects of Siglec-5 on hepatocellular carcinoma and the mechanism of action. The interactions and expression changes between Siglec-5 and Siglec-14 not only affect immune cell function, but may also influence tumor progression. A deeper understanding of the mechanisms regulating their balance could provide new insights and strategies for hepatocellular carcinoma treatment.

Methods

A cell co-culture model was established. Western blotting was used to detect protein expression in different groups. The CCK-8 assay was employed to observe the proliferation of HepG2 hepatocellular carcinoma cells, and the Transwell assay was used to examine their migration. Tumorigenic capacity of HepG2 cells detected by subcutaneous transplantation tumor assay in nud

SUBMITTER: Luo S 

PROVIDER: S-EPMC12381118 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature