LRRC75A-AS1 facilitates breast cancer cell proliferation and invasion via functioning as a CeRNA to modulate miR489-3p/ARD1.

ABSTRACT: This study aimed to elucidate the molecular mechanism through which ARD1 regulates breast cancer (BC) progression via the LRRC75A-AS1/miR-489-3p axis. The expression levels of ARD1, miR-489-3p, and LRRC75A-AS1 in BC cells were quantified using reverse transcription-polymerase chain reaction (RT-PCR). The interaction between miR-489-3p and ARD1 was validated through dual-luciferase reporter assays and RNA-binding protein immunoprecipitation (RIP). The sponge effect of LRRC75A-AS1 on miR-489-3p was confirmed by RNA pull-down assays. Functional roles of LRRC75A-AS1, miR-489-3p, and ARD1 in cell proliferation, invasion, and epithelial-to-mesenchymal transition (EMT) were evaluated using colony formation, Transwell, and western blot assays. Moreover, in vivo tumor xenograft experiments were con

SUBMITTER: Yu C 

PROVIDER: S-EPMC12381120 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature