Application of Brucella type IV secretion proteins VirB1, VirB5, and VirB6 in the serological diagnosis of brucellosis.

ABSTRACT: Brucellosis, a significant zoonotic infectious disease caused by Brucella spp., necessitates the development of efficient, rapid, accurate, and cost-effective diagnostic methods. This study evaluated the diagnostic value of Brucella Type IV secretion proteins VirB1, VirB5, and VirB6 for human brucellosis serological diagnosis. A total of 100 positive serum samples, 96 negative serum samples, and 27 serum samples from patients with fever caused by other pathogens were collected. Recombination VirB1 (rVirB1), rVirB5, and rVirB6 were expressed using prokaryotic expression systems, while VirB2 and VirB7 were synthesized as peptides. An indirect ELISA method was established using purified proteins and synthetic peptides, with sensitivity, specificity, AUC, and cut-off values determined through

SUBMITTER: Wang L 

PROVIDER: S-EPMC12381201 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature