Acute metabolite responses to swimming exercise of different intensities in highly trained male and female swimmers.

ABSTRACT: We investigated metabolite responses to different swimming intensities in 16 highly trained swimmers (9 males, 7 females, aged 16-24 years). After determining critical swimming speed (CS) with a 12 × 25 m maximal effort test, participants completed three swimming trials at moderate (below CS), heavy (at CS), and severe (above CS) intensities on separate days. Capillary blood samples (1 mL) were collected before and after each trial for metabolite profiling via mass spectrometry. Orthogonal partial least squares analysis (OPLS-DA) revealed distinct metabolite changes between moderate and severe intensity trials [R²X (cum): 0.56; R²Y(cum): 0.95, pR²Y: 0.02, pQ²: 0.02]. Free fatty acids (FFAs) 18:0, 20:0, 20:4, and 22:4 showed higher log₂

SUBMITTER: Govus AD 

PROVIDER: S-EPMC12381355 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature