[Microfluidic photo-curing fabrication of silk fibroin/hyaluronic acid composite microsphere hydrogels].

ABSTRACT:

Objectives

To fabricate an injectable composite microsphere hydrogel reinforced with silk fibroin/hyaluronic acid microspheres, achieving synergistic enhance-ment of mechanical robustness and biofunctionality.

Methods

Methacrylated hyaluronic acid (HAMA) and thiolated silk fibroin (TSF) were synthesized. Monodisperse microspheres generated via microfluidics were UV-cured (420 nm) through thiol-ene click reaction. These microspheres were embedded in a TSF/HAMA matrix to form photo-cured composites. The grafting rate of TSF and HAMA was characterized by H1-NMR; particle size distribution of microsphere hydrogels in soybean oil was observed by optical microscopy; gel point of composite microsphere hydrogels was determined by advanced extensional rheometer; microscopic morpholog

SUBMITTER: Wang R 

PROVIDER: S-EPMC12382315 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature