Evaluation of Bioactive Compounds, Antioxidant Activity, and Anticancer Potential of Wild Ganoderma lucidum Extracts from High-Altitude Regions of Nepal.

ABSTRACT: Wild Ganoderma lucidum from Nepal's high-altitude regions was studied to identify key bioactive compounds and assess the influence of solvent type-water, ethanol, methanol, and acetone-on extraction efficiency and biological activity. Extracts were evaluated for antioxidant potential, cytotoxicity against HeLa cells, and phytochemical composition via gas chromatography-mass spectrometry (GC-MS). Solvent type significantly affected both yield and bioactivity. Acetone yielded the highest crude extract (5.01%), while ethanol extract exhibited the highest total phenolic (376.5 ± 9.3 mg PG/g) and flavonoid content (30.3 ± 0.5 mg QE/g). Methanol extract was richest in lycopene (0.07 ± 0.00 mg/g) and β-carotene (0.45 ± 0.02 mg/g). Ethanol extract demonstrated consistently strong DPPH, superoxide,

SUBMITTER: Thapa I 

PROVIDER: S-EPMC12384079 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature