Engineered TtgR-Based Whole-Cell Biosensors for Quantitative and Selective Monitoring of Bioactive Compounds.

ABSTRACT: TtgR, a transcriptional repressor from Pseudomonas putida, plays a key role in regulating multidrug resistance by controlling the expression of genes in response to various ligands. Despite its broad specificity, TtgR represents a promising candidate for the development of transcription factor (TF)-based biosensors. In this study, we utilized TtgR and its native promoter region (P_ttgABC) as genetic components to construct TF-based biosensors in Escherichia coli. By coupling TtgR and P_ttgABC with egfp, we developed a biosensor responsive to diverse flavonoids. To enhance the selectivity and specificity of the biosensor, we genetically engineered a TtgR-binding pocket. Engineered TtgR variants exhibited altered sensing profiles, enabli

SUBMITTER: Song K 

PROVIDER: S-EPMC12384351 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature