Methodological Development of a Test for Salivary Proteome Analysis Useful in Lung Cancer Screening.

ABSTRACT: Early diagnosis of lung cancer, essential for reducing its high mortality rate, is currently challenging, partly due to the lack of specific biomarkers. Here, we attempted to develop a noninvasive and potentially sensitive screening method based on the proteomic analysis of unstimulated and stimulated saliva samples, collected by passive drooling and salivary swabs, respectively, from healthy heavy smokers enrolled in a nonprofit screening project. Protein content analyzed before and after sample cryopreservation for various periods and the associated two-dimensional electrophoresis revealed that protein extraction after short-term cryopreservation prevented the loss of detectable proteins. Mass spectrometric analysis of these electrophoretically resolved proteins revealed the presence of

SUBMITTER: Barra L 

PROVIDER: S-EPMC12386888 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature