Isolation and Purification of Novel Antioxidant Peptides from Mussel (<i>Mytilus edulis</i>) Prepared by Marine <i>Bacillus velezensis</i> Z-1 Protease.

ABSTRACT: Mussels are nutrient-rich but perishable, resulting in substantial resource loss. A protease-producing strain (Bacillus velezensis Z-1, Mytilus edulis) isolated from marine sludge was used to hydrolyze mussels, producing Y-1, a hydrolysate with antioxidant activity. In this study, ultrafiltration, gel chromatography, and LC-MS/MS were employed to isolate and identify bioactive peptides from the hydrolysate. The results revealed that the hydrolysate exhibited antioxidant activity, pancreatic cholesterol esterase inhibitory activity, pancreatic lipase inhibitory activity, and α-glucosidase inhibitory activity. Molecular docking using AutoDock Tools 1.5.6 was performed to analyze the interactions of peptides with CD38 and Keap1, leading to the identification of five potentially

SUBMITTER: Lu J 

PROVIDER: S-EPMC12387240 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature