Evaluating the Assembly Strategy of a Fungal Genome from Metagenomic Data: <i>Solorina crocea</i> (Peltigerales, Ascomycota) as a Case Study.

ABSTRACT: The advent of next-generation sequencing technologies has given rise to considerably diverse techniques. However, integrating data from these technologies to generate high-quality genomes remains challenging, particularly when starting from metagenomic data. To provide further insight into this process, the genome of the lichenized fungus Solorina crocea was sequenced using DNA extracted from the thallus, which contains the genome of the mycobiont, along with those of the photobionts (a green alga and a cyanobacterium), and other associated microorganisms. Three different strategies were assessed for the assembly of a de novo genome, employing data obtained from Illumina and PacBio HiFi technologies: (1) hybrid assembly based on metagenomic data; (2) assembly based on metagen

SUBMITTER: Garcia-Munoz A 

PROVIDER: S-EPMC12387558 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature