Membrane Stress Enhances Specific PQS-Lipid Interactions That Drive Bacterial Outer Membrane Vesicle Biogenesis.

ABSTRACT: Gram-negative bacteria use outer membrane vesicles (OMVs) for toxin trafficking, immune interference, horizontal gene transfer, antibiotic protection, and cell-cell communication. Despite their direct contribution to many pathogenesis-related behaviors, our understanding of how OMVs are produced remains surprisingly incomplete. The Bilayer Couple model describes the induction of OMV formation resulting from the preferential accumulation of small molecules in the outer leaflet of the membrane, resulting in leaflet expansion and membrane bending. Previous work has highlighted the importance of the structure of the Pseudomonas Quinolone Signal (PQS) in driving OMV formation, but the nature of interactions with membrane lipids remains unclear. Our recent in silico analysis suggested that a new

SUBMITTER: Gopal C 

PROVIDER: S-EPMC12388362 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature