Genetic and Evolutionary Analysis of Porcine Kobuvirus in Guangxi Province, Southern China, Between 2021 and 2025.

ABSTRACT: Kobuvirus is a new genus of viruses in the Picornaviridae family causing diarrhea in animals. Porcine kobuvirus (PKV) is an important pathogen with a high rate of infection in pig herds. In this study, a total of 10,990 fecal swabs and tissue samples were collected from different areas of Guangxi province in southern China during 2021-2025 and then tested for PKV using RT-qPCR. The results showed a 19.19% (2109/10,990) PKV positivity rate. Sixty-two PKV-positive samples, which were selected according to sampling regions, sampling seasons, and detection Ct values, were used for PCR amplification and gene sequencing. A sequence comparison showed that the nucleotide and amino acid identities of VP1, 2B, and 3D genes were 78.6-99.5% and 83.5-100%, 77.7-99.8% and 80.

SUBMITTER: Tang Y 

PROVIDER: S-EPMC12388615 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature