Heatwave-Induced Thermal Stratification Shaping Microbial-Algal Communities Under Different Climate Scenarios as Revealed by Long-Read Sequencing and Imaging Flow Cytometry.

ABSTRACT: The effect of periodical heatwaves and related thermal stratification in freshwater aquatic ecosystems has been a hot research issue. A large dataset of samples was generated from samples exposed to temporary thermal stratification in mesocosms mimicking shallow eutrophic freshwater lakes. Temperature regimes were based on IPCC climate warming scenarios, enabling simulation of future warming conditions. Surface oxygen levels reached 19.37 mg/L, while bottom layers dropped to 0.07 mg/L during stratification. Analysis by FlowCAM revealed dominance of Cyanobacteria under ambient conditions (up to 99.2%), while Cryptophyta (up to 98.9%) and Chlorophyta (up to 99.9%) were predominant in the A2 and A2+50% climate scenarios, respectively. We identified temperature changes and shifts in nutrient c

SUBMITTER: Meirkhanova A 

PROVIDER: S-EPMC12389774 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature