<i>Microcystis aeruginosa msoT1/msoA1</i> Locus Displays Features of a Type I Toxin-Antitoxin System.

ABSTRACT: Type I toxin-antitoxin (TA) systems consist of a protein toxin that exerts a cytostatic or cytotoxic effect and an antisense RNA antitoxin that prevents translation of the toxin. Although well studied, type I TA systems have so far only been discovered in bacteria from the phyla Proteobacteria, Firmicutes, and Tenericutes. We hypothesized that type I systems could also be present in Cyanobacteria. Through bioinformatic analysis of the Microcystis aeruginosa PCC 7806SL genome, we discovered ten putative type I TA loci and characterized six of them experimentally. Two of the six putative type I toxins, BH695_0320 and MsoT1 (BH695_4017), were observed to negatively affect Escherichia coli cell growth, with MsoT1 exerting a phenotype similar to SrnB, a known type I toxin. We focu

SUBMITTER: Ruparcic M 

PROVIDER: S-EPMC12390103 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature