Barcoding Quantitative PCR Assay to Distinguish Between <i>Aedes aegypti</i> and <i>Aedes sierrensis</i>.

ABSTRACT: The accurate identification of mosquito species is critical for effective mosquito surveillance and control, especially when presented with morphologically similar species like Aedes aegypti and Aedes sierrensis. Damaged specimens and morphologically similar life stages such as eggs and larvae make it difficult to distinguish Aedes aegypti from Aedes sierrensis using microscopy and taxonomic keys. To address this, the AegySierr.ID-qPCR assay, a multiplex quantitative PCR assay that utilizes single-nucleotide polymorphisms within the mitochondrial cytochrome oxidase subunit I gene, was developed to distinguish between these two species. The assay was tested on DNA extracted from the eggs, larvae, and adults of both species, as well as from environme

SUBMITTER: Barretto M 

PROVIDER: S-EPMC12390609 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature