Dynamics of bacterial biofilm development imaged using light sheet fluorescence microscopy.

ABSTRACT: Biofilm formation exacerbates bacterial infections and interferes with industrial processes. However, the dynamics of biofilm development, especially if formed by a combination of more than one species, is not entirely understood. Here, we present a microfluidic cultivation system that enables continuous imaging of biofilm growth using light sheet fluorescence microscopy (LSFM). We studied the development of biofilms of the human pathogens Staphylococcus aureus and Pseudomonas aeruginosa. Multidirectional LSFM imaging enables the calculation of a three-dimensional reconstruction of the biofilm structure with isotropic resolution. Whereas S. aureus forms 50-70-μm-thick mushroom-like structures, a P. aeruginosa biofilm is 10-15 μm thick with cell clusters 25 μm in

SUBMITTER: Smerdova L 

PROVIDER: S-EPMC12390859 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature