Optical Redox Imaging Predicts Post-Loading Cartilage Mitochondrial Membrane Potential.

ABSTRACT:

Purpose

Disrupted cellular redox balance is associated with various diseases, including osteoarthritis. Although mitochondrial (MT) membrane potential is a proxy for redox balance, the translational potential of this method is limited by exogenous dye. Therefore, the objective of this study was to predict changes in MT membrane potential in response to mechanical loading using dye-free optical redox imaging (ORI). A secondary objective was to determine the effect of loading on ORI metrics.

Methods

Full-thickness porcine cartilage strips were subjected to tensile loading at one of two strain rates (1.00 s^-1 or 0.10 s^-1). ORI was done before, immediately after, and 30 minutes after loading. MT membrane potential was then measured using fluorescent dye. A

SUBMITTER: Wang J 

PROVIDER: S-EPMC12390869 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature