Syncytial death mediated by oncolytic rVSV-NDV dynamically activates immunogenic apoptosis and necroptosis in human lung cancer cells.

ABSTRACT: Recent work has indicated that oncolytic virotherapy leads to immunogenic cell death (ICD) as an important mechanism of action; however, the underlying cell death pathways leading to ICD have been less explored. Our previous data demonstrated that chimeric oncolytic recombinant vesicular stomatitis virus-Newcastle disease virus (rVSV-NDV) has a strong immune-stimulating potential that seems to be mediated by immunogenic syncytial oncolysis. In this work, we aimed to investigate the role of apoptosis and necroptosis in mediating syncytial cell death. In human lung cancer cell lines (A549 and H1437), we demonstrate that fusogenic rVSV-NDV and the parental virus, rVSV, both dynamically engage apoptosis and necroptosis signaling to mediate oncolysis. Genetic deletion of key death regulators (c

SUBMITTER: Kortum F 

PROVIDER: S-EPMC12390950 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature