A model-based design strategy to engineer miRNA-regulated detection systems.

ABSTRACT: miRNAs are promising diagnostic biomarkers. These small RNA molecules are always present in the human body but become dysregulated when a person develops certain diseases. Although the detection of these biomarkers in cell-free tests is ongoing work, current systems often focus solely on detecting the presence or absence of a specific miRNA, rather than the miRNAs concentration. Thus, these tests may miss relative changes in miRNA concentration when disease-induced dysregulation occurs. This work, part of the WUR iGEM 2024 project (miRADAR), aimed to address this gap by incorporating an miRNA concentration-dependent threshold mechanism in a cell-free diagnostic test. In this system, continuous miRNA input concentrations need to be converted into a binary output signal, classifying the miRN

SUBMITTER: Verkuijlen RJ 

PROVIDER: S-EPMC12390972 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature