Targeting macrophages and ion homeostasis in T2D: new genes and therapeutic pathways identified.

ABSTRACT:

Introduction

Type 2 diabetes (T2D) is characterized by insulin resistance and chronic inflammation, with macrophages playing a crucial role in pancreatic islet dysfunction. This study explored the intersection of macrophage-specific gene expression and abnormal blood monovalent inorganic cation concentration-related genes (ABRGs) in T2D patients via single-cell RNA sequencing (scRNA-seq) and machine learning to identify key genes and potential therapeutic targets.

Methods

ScRNA-seq data from the pancreatic islet cells of 27 nondiabetic (ND) patients and 17 T2D patients were analyzed to identify differentially expressed genes (DEGs) in macrophages. These DEGs were intersected with ABRGs to identify hub genes. Machine learning models were developed to predict T2D, and structur

SUBMITTER: Mou L 

PROVIDER: S-EPMC12391075 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature