Extensive profiling of a complex microbial community by high-throughput sequencing.

ABSTRACT: Complex microbial communities remain poorly characterized despite their ubiquity and importance to human and animal health, agriculture, and industry. Attempts to describe microbial communities by either traditional microbiological methods or molecular methods have been limited in both scale and precision. The availability of genomics technologies offers an unprecedented opportunity to conduct more comprehensive characterizations of microbial communities. Here we describe the application of an established molecular diagnostic method based on the chaperonin-60 sequence, in combination with high-throughput sequencing, to the profiling of a microbial community: the pig intestinal microbial community. Four libraries of cloned cpn60 sequences were generated by two genomic DNA extraction procedu

SUBMITTER: Hill JE 

PROVIDER: S-EPMC123911 | biostudies-literature | 2002 Jun

REPOSITORIES: biostudies-literature