Exercise-induced Metabolite N-lactoyl-phenylalanine Ameliorates Colitis by Inhibiting M1 Macrophage Polarization via the Suppression of the NF-κB Signaling Pathway.

ABSTRACT:

Background & aims

Although the anti-inflammatory benefits of exercise are well-documented, the specific mechanisms responsible for these advantages remain uncertain. N-lactoyl-phenylalanine (Lac-Phe), a major metabolite produced during exercise, is synthesized through the condensation of lactic acid and phenylalanine, catalyzed by the CNDP2. However, the potential anti-inflammatory properties of Lac-Phe remain poorly understood. This study aimed to investigate the anti-inflammatory effects of Lac-Phe in the context of inflammatory bowel disease (IBD) and to examine the underlying mechanisms.

Methods

The levels of Lac-Phe were measured in both patients and mice with IBD utilizing enzyme-linked immunosorbent assay kits. The anti-inflammatory effects of Lac-Phe were demonstrate

SUBMITTER: Yu R 

PROVIDER: S-EPMC12391280 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature