TIMELESS deficiency sensitizes thyroid cancer to cisplatin treatment via the DNA damage repair pathway.

ABSTRACT:

Background

The circadian rhythm plays a crucial role in thyroid cancer progression. DNA damage repair (DDR) pathways are closely associated with the sensitivity to cisplatin. However, the role of circadian rhythm genes on cisplatin resistance in thyroid cancer remains undefined.

Methods

Panels of molecular screening were achieved by signaling antibody array. Molecular expressions were established via western blotting and polymerase chain reaction (PCR). Multiple assays (Cell Counting Kit-8, colony formation, Transwell) were used to assess cellular dynamics. The xenograft tumor model was employed for in vivo therapeutic evaluation.

Results

BCPAP and KTC-1 cells were more resistant to cisplatin than other thyroid cancer cell lines, and DDR pathway activation was detecte

SUBMITTER: Wang C 

PROVIDER: S-EPMC12391424 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature