Extracellular vesicles-delivered circDB promotes ischemic muscle repair through the miR-34a/USP7/Notch1 signaling pathway.

ABSTRACT:

Introduction

The incidence of lower limb ischemic diseases has been rising steadily in recent years, often leading to severe outcomes such as limb amputation. Given the limited availability of effective treatments, there is a critical need for novel therapeutic strategies. This study explores the reparative role and underlying mechanisms of extracellular vesicles derived from human umbilical cord mesenchymal stem cells (UMSC-EVs) in promoting ischemic hindlimb recovery through the delivery of circular RNA circDB.

Methods

A hindlimb ischemia model was established in C57BL/6 mice via femoral artery ligation, followed by intramuscular injections of extracellular vesicles derived from either untreated UMSCs (NC-EVs) or UMSCs transfected with si-circDB (si-EVs). Functional

SUBMITTER: Jiao L 

PROVIDER: S-EPMC12391560 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature