Dual probe ligation in situ hybridization with rolling-circle amplification for high-plex spatial transcriptomics.

ABSTRACT: New biological insights are increasingly dependent upon a deeper understanding of tissue architectures. Critical to such studies are spatial transcriptomics technologies, especially those amenable to analysis of the most widely available human tissue type, formalin-fixed and paraffin-embedded (FFPE) clinical specimens. Here we build on our previous oligonucleotide probe ligation-based approach to accurately analyze FFPE mRNA, which suffers from variable levels of degradation. Ligation In Situ Hybridization followed by rolling circle amplification (LISH-Lock'n'Roll or LISH-LnR), provides a streamlined method to detect the spatial location of specific mRNA isoforms within FFPE tissue architectures. Iterative fluorescent probe hybridization and imaging enables highly multiplexed spatial trans

SUBMITTER: Maguire SE 

PROVIDER: S-EPMC12391569 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature