Printing and Rerouting of Elastic and Protease Responsive Shape Memory Hydrogel Filaments.

ABSTRACT: The fabrication of mechanically robust and reconfigurable hydrogel filaments remains a major challenge in biofabrication of perfusable architectures, dynamic tissue models, and complex 3D cell-laden constructs. Conventional extrusion-based bioprinting techniques generate filaments that are soft and fragile, limiting post-processing, scalability, and functional adaptability. Rerouting of Free-Floating Suspended Hydrogel Filaments (REFRESH) is introduced as a biofabrication strategy that integrates an aqueous two-phase system (ATPS)-compatible elastic extracellular matrix mimicking bioink material with a flexible printing and post-processing approach to overcome these constraints. This method enables the formation of highly elastic hydrogel filaments cross-linked via strain-promoted azide-al

SUBMITTER: Lifwergren P 

PROVIDER: S-EPMC12391619 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature