Animal-Free Setup of a 3D Mature Adipocyte-Macrophage Co-Culture to Induce Inflammation In Vitro.

ABSTRACT: Adipose tissue inflammation plays a central role in the pathogenesis of metabolic disorders. It is closely associated with immune cell infiltration, particularly macrophages, and the release of pro-inflammatory cytokines. Reliable in vitro test systems that mimic the inflamed environment while being free of animal-derived components are essential to explore new treatments for obesity-related diseases. This study aims to develop a straightforward, animal-free adipocyte-macrophage co-culture for investigating adipose tissue inflammation. Therefore, the human monocytic cell lines Mono Mac (MM6) and THP-1 are co-cultured with human primary mature adipocytes (ACs) encapsulated in gellan gum (GG) within a defined environment. Both monocytic cell lines are effectively activated by phorbol 12-myri

SUBMITTER: Nowakowski S 

PROVIDER: S-EPMC12391635 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature