Detection of Anti-IFNγ Autoantibodies From Dried Blood Spots.

ABSTRACT:

Background

High titer, neutralizing anti-cytokine autoantibodies (ACAA) to interferon gamma (IFNγ) are an emerging cause of adult-onset immunodeficiency, and detection can direct clinical management. Currently, the detection of ACAAs is performed on blood or plasma, which is time-sensitive, relatively expensive to obtain, and must be shipped from remote locations in specialized containers. We have adapted an easier method of collecting blood or plasma onto a paper card as dried blood spots (DBS), which is simple, inexpensive, and does not require a medical facility or special mailing.

Methods

Whole blood, either from venipuncture or finger stick, was blotted onto filter paper to yield DBS from which samples were eluted. Samples from 4 groups were analyzed: patients with anti

SUBMITTER: Harris AJ 

PROVIDER: S-EPMC12391752 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature