Rapid, Amplification-free, RNA-based Uropathogen Detection With LbuCas13a.

ABSTRACT:

Background

Expeditious identification of bacterial infection remains an important challenge in an emergency department. Bacterial cultures remain the gold standard, though they take 24-72 hours to result. Polymerase chain reaction-based diagnostics are emerging but take several hours to get a result. Here, we report a rapid bacterial RNA detection platform based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology with urine-to-result time within 1 hour.

Methods

All CRISPR experiments were conducted as an open format plate reader assay with fluorescent readouts. In derivation studies, Escherichia coli 16s rRNA was spiked in commercially purchased human urine to determine assay compatibility and limit of detection. In validation studies, pre

SUBMITTER: Ata H 

PROVIDER: S-EPMC12391756 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature