CK1ε/SRSF10 axis regulates the alternative splicing of Bcl-x in lung cancer cells.

ABSTRACT: The dysregulation of Bcl-x alternative splicing is associated with tumor development and chemoresistance. However, the underlying molecular mechanisms of Bcl-x splicing are still not well-defined. Here, we demonstrated that casein kinase 1ε (CK1ε) was involved in the regulation of Bcl-x alternative splicing. Initially, we noted that SR3029, a specific CK1δ/ε inhibitor, effectively reduced the mRNA and protein expression of Bcl-xL and accompanied by an increase in the mRNA and protein levels of Bcl-xS in a dose-dependent manner. Overexpression of CK1ε decreased the ratio of Bcl-xS/Bcl-xL mRNA and protein compared to the control cells, while depletion of CK1ε leads to an increase in the ratio of Bcl-xS/Bcl-xL. The overexpression of CK1ε also abrogated the impact of serine/arginine-rich splic

SUBMITTER: Sun Q 

PROVIDER: S-EPMC12391806 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature