Polyploid Formation Through Disrupting Mitotic Sister Chromatid Separation of Spermatogonia based on espl1 Heterozygous Knockout in Fish.

ABSTRACT: Polyploidy is a major driver of speciation and evolutionary changes in plants and animals. Production of unreduced gametes is considered as a main pathway for polyploid formation. However, the precise molecular mechanisms underlying unreduced gamete production, particularly those arising from mitotic defects of spermatogonia (SG)/oogonia, remain poorly understood. Here, a heterozygous espl1 (extra spindle pole bodies like 1) knockout line was generated in diploid loach (Misgurnus anguillicaudatus) by CRISPR/Cas9 technology. Interestingly, we found about 90% diploids and 10% triploids in the progeny of espl1+/- males and wild-type (WT) diploid females. espl1+/- male could produce 1n sperms, along with a certain volume of unreduced sperms (2n). All offsprings of espl1+/-♀ and espl1+/+♂ were

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12391874 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature