A Phosphopantetheinyl Transferase from <i>Dictyobacter vulcani</i> sp. W12 Expands the Combinatorial Biosynthetic Toolkit.

ABSTRACT: The value of microbial natural product pathways extends beyond the chemicals they produce, as the enzymes they encode can be harnessed as biocatalysts. Microbial type II polyketide synthases (PKSs) are particularly noteworthy, as these enzyme assemblies produce complex polyaromatic pharmacophores. Combinatorial biosynthesis with type II PKSs has been described as a promising route for accessing never-before-seen bioactive molecules, but this potential is stymied in part by the lack of functionally compatible noncognate proteins across type II PKS systems. Acyl carrier proteins (ACPs) are central to this challenge, as they shuttle reactive intermediates and malonyl building blocks between the other type II PKS domains during biosynthesis. To perform this essential role within PKSs, ACPs mus

SUBMITTER: Hsu KK 

PROVIDER: S-EPMC12391972 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature