CircPLK1 upregulates ETS1 to confer anthracycline resistance in triple-negative breast cancer.

ABSTRACT:

Background and objectives

Circular RNAs play a vital role in developing triple-negative breast cancer (TNBC). Likewise, the function of circRNAs in TNBC resistance to chemotherapy remains largely unknown. Here, we aimed to investigate whether circPLK1 has a biological efect on anthracycline resistance in TNBC.

Methods

We identified circPLK1-a circRNA-using a circRNA microarray in TNBC cells and paired TNBC samples. We assessed the role of circPLK1 in anthracycline resistance in TNBC. Cytotoxicity assay, colony formation assay, and flow cytometry were performed as functional experiments. Western blot analysis, qRT-PCR, in situ hybridization, and immunohistochemistry were used to evaluate the expression of circPLK1, miR-940, and ETS1. RNA immunoprecipitation and lucifer

SUBMITTER: Dai D 

PROVIDER: S-EPMC12392080 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature