Label-Free Quantitative Proteomic Analysis Reveals the Effects of Biogenic Silver Nanoparticles on <i>Fusarium keratoplasticum</i> and Their Therapeutic Potential in <i>Galleria mellonella</i> Larvae.

ABSTRACT: Antifungal drug resistance is a growing concern, necessitating new therapeutic alternatives. This study evaluated the antifungal activity and molecular effects of biogenic silver nanoparticles (AgNPs) synthesized using the culture filtrate of Epicoccum nigrum against Fusarium keratoplasticum, a highly resistant fungal species. AgNPs exhibited strong antifungal activity, with a MIC₅₀ of 1.79 μg/mL and 92.85% growth inhibition at 5.92 μg/mL. Label-free quantitative proteomic analysis (LFQ-MS) revealed 52 proteins with significantly altered abundance after AgNP treatment, affecting the oxidative stress response, mitochondrial function, and riboflavin biosynthesis. Decreased levels of proteins involved in riboflavin biosynthesis and electron transport suggest metabolic

SUBMITTER: Rigotto-Caruso G 

PROVIDER: S-EPMC12392174 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature