Low-Dose Elemental Mapping of Light Atoms in Liquid-phase Materials Using Cryo-EELS.

ABSTRACT: Cryogenic transmission electron microscopy (cryo-TEM) enables the visualization of liquid-phase materials, including nanoparticles and soft/biomaterials, under cryogenic conditions while minimizing radiation damage. Cryo-TEM imaging provides insights into particle size, shape, and dispersion. Beyond such conventional structural information, acquiring elemental composition data allows for a more detailed analysis and evaluation. In this study, we developed a method for elemental mapping of nanoparticles and soft/biomaterials in frozen solvent by integrating electron energy loss spectroscopy (EELS) with energy-filtered (EF) cryo-EM. Cryo-EELS and analysis were performed using the three-window method, following the alignment and correction of stage drift during image acquisition and interexpo

SUBMITTER: Unabara D 

PROVIDER: S-EPMC12392256 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature