Rapid High-Resolution Analysis of Polysaccharide-Lignin Interactions in Secondary Plant Cell Walls Using Proton-Detected Solid-State NMR.

ABSTRACT: The plant secondary cell wall, a complex matrix composed of cellulose, hemicellulose, and lignin, is crucial for the mechanical strength and water-proofing properties of plant tissues, and serves as a primary source of biomass for biorenewable energy and biomaterials. Structural analysis of these polymers and their interactions within the secondary cell wall has been heavily relying on ¹³C-based solid-state NMR techniques. In this study, we explore the application of ¹H-detected solid-state NMR techniques for rapid, high-resolution structural characterization of polysaccharides and lignin, demonstrated on the stems of hardwood eucalyptus. We explored the use of synthesized 2D spectra to resolve central ¹H resonances and the combined application of 3D hCCH a

SUBMITTER: Xiao P 

PROVIDER: S-EPMC12392260 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature